肺臟,肝臟,脾臟三個部位經常是動物實驗需要檢測的部位,如何通過流式檢測肺臟,肝臟,脾臟三個部位的巨噬細胞?首要的是流式圈門,肺肝脾巨噬細胞圈門策略文獻參考,可以參考如下文獻。
論文信息:
論文題目:Microbiota-induced EI24 improves homeostasis but impedes function of alveolar macrophages via metabolic regulation
期刊名稱:Nature Communications
時間期卷:17, Article number: 2227(2026)
在線時間:2026年1月31日
DOI: doi.org/10.1038/s41467-026-68466-5
Clodronate Liposomes氯膦酸鹽脂質體在小鼠病毒感染和腫瘤轉移模型清除肺泡巨噬細胞和外周單核巨噬細胞。荷蘭Liposoma巨噬細胞清除劑ClodronateLiposomes見刊于Nature Communications:微生物群誘導的EI24通過代謝調控改善穩態,但阻礙肺泡巨噬細胞功能。

肺肝脾巨噬細胞圈門策略文獻參考:


a/肺臟:Gating strategy used for identification of lung AMs and IMs.
AMs were identified as CD45+ CD11b1o CD11c+ F4/80+ SiglecF+
IMs were characterized as CD45+CD11b+CD11c- F4/80+.
b/肝臟: Gating strategy of liver macrophages.
Liver macrophages wereidentified as CD45+ F4/80+ CD11b+
c/脾臟: Gating strategy of spleen RPMs.
RPMs wereidentified as CD45+ F4/80+ CD11bint